Understanding differences in streptavidin-biotin binding
Understanding differences in streptavidin-biotin binding
7 months ago
illinois.edu
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Understanding differences in streptavidin-biotin binding
Understanding differences in streptavidin-biotin binding
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SOLVED: Biotin, a B vitamin, bonds tightly to the bacterial protein streptavidin. Researchers measured the strength of the biotin-streptavidin bond by attaching a strand of DNA to a biotin molecule that was bonded to a tethered streptavidin molecule. DNA in solution is charged, with a charge of -2e per base pair. An applied electric field exerts a force on the DNA, and researchers increased the field strength until the biotin-streptavidin bond broke. A 4.2 x 10^4 (N)/(C) field broke the bond whe
SOLVED: Biotin, a B vitamin, bonds tightly to the bacterial protein stre…
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