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🧬 Immunohistochemistry (IHC) 101 — Part 1 The process in a nutshell IHC helps us identify specific antigens in tissue sections using antibodies. Basic flow: 🔹 Fixation & processing 🔹 Antigen retrieval 🔹 Primary antibody binding 🔹 Detection & visualization 🔹 Interpretation ⚠️ But what if the IHC stain goes wrong? ❌ Weak or no staining ❌ High background / nonspecific staining ❌ False positives or false negatives Most issues trace back to pre-analytical errors, antibody selection, retrieval m
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🧬 Immunohistochemistry (IHC) 101 — Part 1 The process in a nutshell IHC helps us identify specific antigens in tissue sections using antibodies. Basic flow: 🔹 Fixation & processing 🔹 Antigen retrieval 🔹 Primary antibody binding 🔹 Detection & visualization 🔹 Interpretation ⚠️ But what if the IHC stain goes wrong? ❌ Weak or no staining ❌ High background / nonspecific staining ❌ False positives or false negatives Most issues trace back to pre-analytical errors, antibody selection, retrieval m
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🌟 Understanding the Role of IHC in Cancer Diagnosis 🌟 A patient came to us with lesions in the ovary, gall bladder, and appendix. A biopsy from the appendix revealed a low-grade mucinous neoplasm, but the primary origin of the tumor was unclear. Using IHC (Immunohistochemistry), we determined whether the tumor originated from the GI tract or pancreatic-biliary tract. Based on these insights, the patient was referred to an onco-surgeon for cytoreductive surgery and HIPEC. Post-op histopathology
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🌟 Understanding the Role of IHC in Cancer Diagnosis 🌟 A patient came to us with lesions in the ovary, gall bladder, and appendix. A biopsy from the appendix revealed a low-grade mucinous neoplasm, but the primary origin of the tumor was unclear. Using IHC (Immunohistochemistry), we determined whether the tumor originated from the GI tract or pancreatic-biliary tract. Based on these insights, the patient was referred to an onco-surgeon for cytoreductive surgery and HIPEC. Post-op histopathology
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