In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Light that is reflected back from the edge of the medium it is traveling through. When light rays travel at an angle greater than the "critical" angle, which is determined by the medium, the light ...

TIRFM is a sophisticated fluorescent microscope that allows a user to visualize a very small region of a specimen. TIRFM utilizes optics to produce light at the interface of the slide. This technique ...

Bound states in the continuum (BICs) are a special class of resonant states. Although they have the same momentum and energy as the propagating modes in free space, yet they are not coupled to the ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

Lab-grown muscle stem cells from mice mimic the formation of muscle fibers in vivo. A series of rodent experiments showed that even with abundant food and water, personal space is essential to prevent ...